Thin Layer Chromatography (TLC), like all chromatographic techniques, based on multi-stage distribution process involving; Although the TLC principle is known more than 100 years, today TLC has gained increasing importance. Applicability of TLC was enhanced by development of new absorbants and supports. The basic steps of TLC are the following; Sample preparation. Sample application. Development of chromatogram. Evaluation in TLC. Silica 60, specific surface (BET) - 500 m2/g, mean pore size 60A, specific pore volume 0,75 ml/g, particle size 5 - 17 µm:standard grade. Thickness of layer for analytical plates 0,25 mm. For preparative plates 0,5 and 1,0 mm. For 2,0 mm preparative layers a slightly closer material is used. Indicators: manganese activated zinc silicate with green fluorescence for short-wave UV ( 254 nm ); special inorganic fluorescent pigment with blue fluorescence for long-wave UV (366 nm). Binders: highly polimeric products, which are stable in almost all organic solvents and resistant towards aggressive visualisation reagents. Silica 60, specific surface (BET) - 500 m2/g, mean pore size 60A, specific pore volume 0,75 ml/g, particle size 5 - 17 µm standard grade. Binders: highly polimeric products, which are stable in almost all organic solvents and resistant towards aggressive visualisation reagents, also completely stable in purely aqueous eluents.

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